Regulatory
Part:BBa_J100066:Design
Designed by: Rebecca Evans Group: Campbell M Lab (2012-06-12)
Synthetic Riboswitch
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 196 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 197
Illegal PstI site found at 211
Illegal NotI site found at 7
Illegal NotI site found at 204 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 197 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 197
Illegal PstI site found at 211
Illegal AgeI site found at 122 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 190
Design Notes
Designed to permit translation in the presence of theophylline.
Source
Synthetic part taken from reference below.
References
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988590/?tool=pubmed